opg sirna Search Results


opg sirna  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology opg sirna
Fig. 7 <t>OPG</t> downregulation inhibits the breast carcinogenesis of breast cancer cells. MDA-MB-231 and BT-20 cells were transfected with OPG <t>siRNA</t> (OPG-si), while a scrambled sequence was used as control (Ctrl). A Western blot images with GAPDH used as internal control. The numbers underneath each band represent fold changes relative to the control (Ctrl) after normalization to GAPDH. Phosphorylated protein fractions were determined by quantification and normalization against their relative non-phosphorylated forms (B) Cellular analysis using the real-time xCELLigence RTCA System. C Cells (2 × 105) were harvested and stained with propidium iodide (PI), and were analyzed by flow cytometry. The fractions of cells in each phase of the cell cycle are depicted in the boxes. D Cells (1 × 103) were seeded in ultra-low attachment 96‐well plate containing stem cell‐specific medium. The number of spheroids (>100 μm) were counted. Representative photographs of spheroids (left panel), number of formed spheroids (right panel). Error bars represent mean ± SEM (n = 3). *P ≤0.05; **P ≤0.01.
Opg Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/opg sirna/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
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murine opg targeting sirnas for opg  (Ribobio co)
90
Ribobio co murine opg targeting sirnas for opg
Skeletal stem cells (SSCs) suppress inflammatory osteoclast (OC) formation through secreted osteoprotegerin <t>(OPG).</t> SSCs (2 × 10 3 SSCs per well) were cultured in the lower compartment of Transwell chambers with a 0.4‐μm‐pore‐size membrane, with CD11b + monocytes (2 × 10 3 CD11b + per well) in the upper compartment. SSC maintained the inhibitory effects without cell‐cell contact. Bars represent 200 μm. B, The tartrate‐resistant acid phosphatase positive (TRAP+) osteoclast formation partially recovered. ** P < .01, compared with no‐Transwell group, n = 5. In addition, (C) OPG mRNA expression in SSCs and (D) SSC‐secreted OPG were detected by quantitative PCR and ELISA, respectively. Both mRNA expression and protein secretion by SSCs were remarkably elevated in the presence of human osteoarthritis synovial fluid (OASF). * P < .05; ** P < .01, compared with no‐OASF group, n = 6. E, Furthermore, the TRAP + osteoclast formation in the SSC/OC coculture system (2 × 10 3 SSC and 2 × 10 3 CD11b + monocytes per well) was partially rescued when OPG in SSC were blocked with <t>OPG‐targeted</t> <t>RNAi</t> and (F) an anti‐OPG‐neutralization antibody (200 ng/mL). * P < .05, compared with NC groups, n = 6
Murine Opg Targeting Sirnas For Opg, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/murine opg targeting sirnas for opg/product/Ribobio co
Average 90 stars, based on 1 article reviews
murine opg targeting sirnas for opg - by Bioz Stars, 2026-03
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lv-opg-rnai-1 vector which contained small interfering rna (sirna) targeting opg expression  (Shanghai Genechem Ltd)
90
Shanghai Genechem Ltd lv-opg-rnai-1 vector which contained small interfering rna (sirna) targeting opg expression
Skeletal stem cells (SSCs) suppress inflammatory osteoclast (OC) formation through secreted osteoprotegerin <t>(OPG).</t> SSCs (2 × 10 3 SSCs per well) were cultured in the lower compartment of Transwell chambers with a 0.4‐μm‐pore‐size membrane, with CD11b + monocytes (2 × 10 3 CD11b + per well) in the upper compartment. SSC maintained the inhibitory effects without cell‐cell contact. Bars represent 200 μm. B, The tartrate‐resistant acid phosphatase positive (TRAP+) osteoclast formation partially recovered. ** P < .01, compared with no‐Transwell group, n = 5. In addition, (C) OPG mRNA expression in SSCs and (D) SSC‐secreted OPG were detected by quantitative PCR and ELISA, respectively. Both mRNA expression and protein secretion by SSCs were remarkably elevated in the presence of human osteoarthritis synovial fluid (OASF). * P < .05; ** P < .01, compared with no‐OASF group, n = 6. E, Furthermore, the TRAP + osteoclast formation in the SSC/OC coculture system (2 × 10 3 SSC and 2 × 10 3 CD11b + monocytes per well) was partially rescued when OPG in SSC were blocked with <t>OPG‐targeted</t> <t>RNAi</t> and (F) an anti‐OPG‐neutralization antibody (200 ng/mL). * P < .05, compared with NC groups, n = 6
Lv Opg Rnai 1 Vector Which Contained Small Interfering Rna (Sirna) Targeting Opg Expression, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lv-opg-rnai-1 vector which contained small interfering rna (sirna) targeting opg expression/product/Shanghai Genechem Ltd
Average 90 stars, based on 1 article reviews
lv-opg-rnai-1 vector which contained small interfering rna (sirna) targeting opg expression - by Bioz Stars, 2026-03
90/100 stars
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opg sirna  (Shanghai GenePharma)
90
Shanghai GenePharma opg sirna
Skeletal stem cells (SSCs) suppress inflammatory osteoclast (OC) formation through secreted osteoprotegerin <t>(OPG).</t> SSCs (2 × 10 3 SSCs per well) were cultured in the lower compartment of Transwell chambers with a 0.4‐μm‐pore‐size membrane, with CD11b + monocytes (2 × 10 3 CD11b + per well) in the upper compartment. SSC maintained the inhibitory effects without cell‐cell contact. Bars represent 200 μm. B, The tartrate‐resistant acid phosphatase positive (TRAP+) osteoclast formation partially recovered. ** P < .01, compared with no‐Transwell group, n = 5. In addition, (C) OPG mRNA expression in SSCs and (D) SSC‐secreted OPG were detected by quantitative PCR and ELISA, respectively. Both mRNA expression and protein secretion by SSCs were remarkably elevated in the presence of human osteoarthritis synovial fluid (OASF). * P < .05; ** P < .01, compared with no‐OASF group, n = 6. E, Furthermore, the TRAP + osteoclast formation in the SSC/OC coculture system (2 × 10 3 SSC and 2 × 10 3 CD11b + monocytes per well) was partially rescued when OPG in SSC were blocked with <t>OPG‐targeted</t> <t>RNAi</t> and (F) an anti‐OPG‐neutralization antibody (200 ng/mL). * P < .05, compared with NC groups, n = 6
Opg Sirna, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/opg sirna/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
opg sirna - by Bioz Stars, 2026-03
90/100 stars
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sirna against opg  (Qiagen)
90
Qiagen sirna against opg
Skeletal stem cells (SSCs) suppress inflammatory osteoclast (OC) formation through secreted osteoprotegerin <t>(OPG).</t> SSCs (2 × 10 3 SSCs per well) were cultured in the lower compartment of Transwell chambers with a 0.4‐μm‐pore‐size membrane, with CD11b + monocytes (2 × 10 3 CD11b + per well) in the upper compartment. SSC maintained the inhibitory effects without cell‐cell contact. Bars represent 200 μm. B, The tartrate‐resistant acid phosphatase positive (TRAP+) osteoclast formation partially recovered. ** P < .01, compared with no‐Transwell group, n = 5. In addition, (C) OPG mRNA expression in SSCs and (D) SSC‐secreted OPG were detected by quantitative PCR and ELISA, respectively. Both mRNA expression and protein secretion by SSCs were remarkably elevated in the presence of human osteoarthritis synovial fluid (OASF). * P < .05; ** P < .01, compared with no‐OASF group, n = 6. E, Furthermore, the TRAP + osteoclast formation in the SSC/OC coculture system (2 × 10 3 SSC and 2 × 10 3 CD11b + monocytes per well) was partially rescued when OPG in SSC were blocked with <t>OPG‐targeted</t> <t>RNAi</t> and (F) an anti‐OPG‐neutralization antibody (200 ng/mL). * P < .05, compared with NC groups, n = 6
Sirna Against Opg, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna against opg/product/Qiagen
Average 90 stars, based on 1 article reviews
sirna against opg - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Fig. 7 OPG downregulation inhibits the breast carcinogenesis of breast cancer cells. MDA-MB-231 and BT-20 cells were transfected with OPG siRNA (OPG-si), while a scrambled sequence was used as control (Ctrl). A Western blot images with GAPDH used as internal control. The numbers underneath each band represent fold changes relative to the control (Ctrl) after normalization to GAPDH. Phosphorylated protein fractions were determined by quantification and normalization against their relative non-phosphorylated forms (B) Cellular analysis using the real-time xCELLigence RTCA System. C Cells (2 × 105) were harvested and stained with propidium iodide (PI), and were analyzed by flow cytometry. The fractions of cells in each phase of the cell cycle are depicted in the boxes. D Cells (1 × 103) were seeded in ultra-low attachment 96‐well plate containing stem cell‐specific medium. The number of spheroids (>100 μm) were counted. Representative photographs of spheroids (left panel), number of formed spheroids (right panel). Error bars represent mean ± SEM (n = 3). *P ≤0.05; **P ≤0.01.

Journal: Cell death discovery

Article Title: Endogenous osteoprotegerin (OPG) represses ERα and promotes stemness and chemoresistance in breast cancer cells.

doi: 10.1038/s41420-024-02151-8

Figure Lengend Snippet: Fig. 7 OPG downregulation inhibits the breast carcinogenesis of breast cancer cells. MDA-MB-231 and BT-20 cells were transfected with OPG siRNA (OPG-si), while a scrambled sequence was used as control (Ctrl). A Western blot images with GAPDH used as internal control. The numbers underneath each band represent fold changes relative to the control (Ctrl) after normalization to GAPDH. Phosphorylated protein fractions were determined by quantification and normalization against their relative non-phosphorylated forms (B) Cellular analysis using the real-time xCELLigence RTCA System. C Cells (2 × 105) were harvested and stained with propidium iodide (PI), and were analyzed by flow cytometry. The fractions of cells in each phase of the cell cycle are depicted in the boxes. D Cells (1 × 103) were seeded in ultra-low attachment 96‐well plate containing stem cell‐specific medium. The number of spheroids (>100 μm) were counted. Representative photographs of spheroids (left panel), number of formed spheroids (right panel). Error bars represent mean ± SEM (n = 3). *P ≤0.05; **P ≤0.01.

Article Snippet: Transfection with OPG siRNA and universal scrambled sequence (30 nM) (Santa Cruz Biotechnology) was performed using the RNAiFect reagent (Qiagen) following the manufacturer recommendations.

Techniques: Transfection, Sequencing, Control, Western Blot, Staining, Cytometry

Skeletal stem cells (SSCs) suppress inflammatory osteoclast (OC) formation through secreted osteoprotegerin (OPG). SSCs (2 × 10 3 SSCs per well) were cultured in the lower compartment of Transwell chambers with a 0.4‐μm‐pore‐size membrane, with CD11b + monocytes (2 × 10 3 CD11b + per well) in the upper compartment. SSC maintained the inhibitory effects without cell‐cell contact. Bars represent 200 μm. B, The tartrate‐resistant acid phosphatase positive (TRAP+) osteoclast formation partially recovered. ** P < .01, compared with no‐Transwell group, n = 5. In addition, (C) OPG mRNA expression in SSCs and (D) SSC‐secreted OPG were detected by quantitative PCR and ELISA, respectively. Both mRNA expression and protein secretion by SSCs were remarkably elevated in the presence of human osteoarthritis synovial fluid (OASF). * P < .05; ** P < .01, compared with no‐OASF group, n = 6. E, Furthermore, the TRAP + osteoclast formation in the SSC/OC coculture system (2 × 10 3 SSC and 2 × 10 3 CD11b + monocytes per well) was partially rescued when OPG in SSC were blocked with OPG‐targeted RNAi and (F) an anti‐OPG‐neutralization antibody (200 ng/mL). * P < .05, compared with NC groups, n = 6

Journal: Stem Cells Translational Medicine

Article Title: Skeletal stem cell‐mediated suppression on inflammatory osteoclastogenesis occurs via concerted action of cell adhesion molecules and osteoprotegerin

doi: 10.1002/sctm.19-0300

Figure Lengend Snippet: Skeletal stem cells (SSCs) suppress inflammatory osteoclast (OC) formation through secreted osteoprotegerin (OPG). SSCs (2 × 10 3 SSCs per well) were cultured in the lower compartment of Transwell chambers with a 0.4‐μm‐pore‐size membrane, with CD11b + monocytes (2 × 10 3 CD11b + per well) in the upper compartment. SSC maintained the inhibitory effects without cell‐cell contact. Bars represent 200 μm. B, The tartrate‐resistant acid phosphatase positive (TRAP+) osteoclast formation partially recovered. ** P < .01, compared with no‐Transwell group, n = 5. In addition, (C) OPG mRNA expression in SSCs and (D) SSC‐secreted OPG were detected by quantitative PCR and ELISA, respectively. Both mRNA expression and protein secretion by SSCs were remarkably elevated in the presence of human osteoarthritis synovial fluid (OASF). * P < .05; ** P < .01, compared with no‐OASF group, n = 6. E, Furthermore, the TRAP + osteoclast formation in the SSC/OC coculture system (2 × 10 3 SSC and 2 × 10 3 CD11b + monocytes per well) was partially rescued when OPG in SSC were blocked with OPG‐targeted RNAi and (F) an anti‐OPG‐neutralization antibody (200 ng/mL). * P < .05, compared with NC groups, n = 6

Article Snippet: For the RNAi assay, the murine OPG targeting siRNAs for OPG and a negative control (NC) were obtained from Ribobio ( http://www.ribobio.com ).

Techniques: Cell Culture, Pore Size, Membrane, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Neutralization